https://firas.phd/tags/note/ Recent content in Note on Firas Sadiyah https://firas.phd/favicon.png https://firas.phd/favicon.png Hugo en Tue, 23 Jan 2024 14:57:54 +0000 https://firas.phd/posts/illumina_sbs/ Sun, 10 Jun 2018 12:00:00 +0000 https://firas.phd/posts/illumina_sbs/ <p>Multiplexing</p> <ul> <li>Index 1 (i7) is always read.</li> <li>Index 2 (i5) is read only in dual index setting.</li> </ul> <table> <thead> <tr> <th style="text-align: center"></th> <th style="text-align: center">Single indexing</th> <th style="text-align: center">Dual indexing</th> </tr> </thead> <tbody> <tr> <td style="text-align: center">Single end (SE)</td> <td style="text-align: center">R1, R2</td> <td style="text-align: center">R1, R2, R3</td> </tr> <tr> <td style="text-align: center">Paired end (PE)</td> <td style="text-align: center">R1, R2, R4</td> <td style="text-align: center">R1, R2, R3, R4</td> </tr> </tbody> </table> <p><img loading="lazy" src="https://firas.phd/images/Illumina_SBS/Illumina_SBS_1.png"></p> <p><img loading="lazy" src="https://firas.phd/images/Illumina_SBS/Illumina_SBS_2.png"></p> <p>Multiplexing - dual index reads - paired end <img loading="lazy" src="https://firas.phd/images/Illumina_SBS/Illumina_SBS_3.png"></p>